中国无机分析化学

水溶性的维生素C是一种人体必需的营养物质。维生素C是含有烯二醇基的多羟基内酯结构,利用其水溶液在波长265nm处有较强烈的紫外吸收峰,建立了直接用紫外分光光度法测定新鲜蔬菜中维生素C含量的方法。实验结果表明,维生素C的含量在1.0~12.0μg/mL内线性关系良好,线性方程为A=0.066 03c+0.019 74(R2=0.997 51),检出限为0.044μg/mL,样品加标回收率为97.5%~105.4%。方法操作简单,准确度高,对新鲜蔬菜的测定结果令人满意。

Soluble vitamin C(Vc)is an essential nutrient for human beings.Vc contains enediol moiety in apolyhydroxy lactone structure A spectrophotometric method for the direct determination of Vc content in fresh vegetables was established,because Vc aqueous solution has relative strong characteristic absorbance at 265 nm.A good linear relationship was obtained in the range of 1.0~12.0μg/mL of the Vc content.The regression equation was A=0.066 03c+0.019 74(R2=0.997 51).The detection limit was 0.044μg/mL with the recoveries of 97.5% ~105.4%.The method was simple and accurate.It can meet the requirements for the determination of Vc in fresh vegetable samples with satisfactory results.